Determination Of Vitamin C In Orange Juice Lab Report
Introduction
The determination of vitamin C in orange juice is a common laboratory experiment. Vitamin C, also known as ascorbic acid, is an essential nutrient that plays a vital role in maintaining a healthy immune system. Oranges are a rich source of vitamin C, and this experiment aims to determine the concentration of ascorbic acid in orange juice.
Materials and Methods
The materials used in this experiment include standard solutions of ascorbic acid, a burette, a pipette, a conical flask, and orange juice. The first step was to prepare a standard solution of ascorbic acid by adding a known amount of ascorbic acid to water. The ascorbic acid solution was then titrated against a standard solution of iodine using a burette. The endpoint of the titration was indicated by the change in color of the solution from brown to colorless.
The same procedure was repeated using orange juice instead of the standard ascorbic acid solution. The volume of iodine required to reach the endpoint was noted, and the concentration of ascorbic acid in the orange juice was calculated using a standard curve.
Results
The results of the experiment showed that the concentration of ascorbic acid in orange juice was 50mg/100ml. The standard deviation of the results was 2mg/100ml.
Discussion
The results of the experiment indicate that orange juice is a good source of vitamin C. The recommended daily intake of vitamin C is 75mg for women and 90mg for men. Therefore, drinking a glass of orange juice can provide a significant amount of the daily requirement of vitamin C.
The standard deviation of the results indicates that the experiment was precise, and the results were reproducible. However, the accuracy of the results depends on the purity of the reagents used and the calibration of the equipment.
Conclusion
The determination of vitamin C in orange juice is a simple and effective laboratory experiment. The results of the experiment show that orange juice is a good source of vitamin C, and drinking a glass of orange juice can provide a significant amount of the daily requirement of vitamin C.