Determination Of Vitamin C Concentration By Titration Lab Report
Introduction
Vitamin C is an essential nutrient that is required by the body to carry out various physiological processes. It acts as an antioxidant, helps in the formation of collagen, aids in the absorption of iron and is important for maintaining the immune system. The recommended daily intake of Vitamin C varies from 65-90mg per day depending on age, gender and other factors.
It is important to measure the concentration of Vitamin C in various food and drink samples as it can help in determining their nutritional value. This can be done by performing a titration experiment.
Materials Required
The following materials are required to carry out the experiment:
- Vitamin C solution
- 2,6-dichlorophenolindophenol (DCPIP) solution
- Sulfuric acid solution
- Distilled water
- Burette
- Pipette
- Erlenmeyer flask
- Conical flask
Methodology
The following steps should be followed to carry out the experiment:
- Prepare a standard solution of DCPIP by dissolving 0.1g of the compound in 50ml of distilled water.
- Add 1ml of sulfuric acid solution to the DCPIP solution and mix well.
- Prepare a Vitamin C solution by dissolving a known amount of the compound in distilled water.
- Fill the burette with the DCPIP solution and titrate it against the Vitamin C solution.
- The endpoint of the titration is reached when the DCPIP solution changes from blue to colorless.
- Note the volume of DCPIP solution used to reach the endpoint.
Calculations
The concentration of Vitamin C in the solution can be calculated using the following formula:
Concentration of Vitamin C (mg/ml) = [(Volume of DCPIP solution used) x (Concentration of DCPIP solution) x (10)] / (Volume of Vitamin C solution taken)
Results
The concentration of Vitamin C in the solution can be determined using the above formula. The results obtained can be tabulated and analyzed.
Conclusion
The determination of Vitamin C concentration by titration is an important experiment that can help in determining the nutritional value of various food and drink samples. The experiment is simple and can be carried out in any laboratory setting. The results obtained can be used to make dietary recommendations and to ensure that individuals are meeting their daily recommended intake of Vitamin C.