Determination Of Iron In Vitamin Tablets By Uv Visible Spectroscopy
Vitamin tablets are an essential part of our daily dietary intake. They are a combination of different vitamins and minerals that help us maintain a healthy lifestyle. One of the most important minerals that we need in our diet is Iron. Iron is an essential mineral that helps in the formation of red blood cells, which carry oxygen to different parts of the body. It also helps in the proper functioning of the immune system and the production of hormones.
Iron deficiency is a common problem worldwide, and people often take iron supplements in the form of vitamin tablets to maintain their iron levels. However, it is essential to monitor the amount of iron in these tablets to ensure that they are safe for consumption.
UV Visible Spectroscopy
UV Visible Spectroscopy is a technique used to determine the concentration of a particular compound in a sample. It involves measuring the absorption of light by the sample over a range of wavelengths. The absorption of light by a compound depends on its chemical structure and concentration in the sample.
In the case of Iron, it absorbs light in the UV visible region, making it suitable for analysis using UV Visible Spectroscopy. The amount of light absorbed by the sample is directly proportional to the concentration of Iron in the sample.
The Procedure
The procedure for determining the amount of Iron in vitamin tablets using UV Visible Spectroscopy is relatively simple. The first step is to prepare a standard solution of Iron by dissolving a known amount of Iron in a solvent.
Next, a sample of the vitamin tablet is dissolved in a solvent, and the absorbance of the sample is measured using a UV Visible Spectrophotometer. The absorbance of the sample is then compared to the absorbance of the standard solution, and the concentration of Iron in the sample is calculated using a calibration curve.
The Calibration Curve
The Calibration Curve is a plot of the absorbance of a standard solution of Iron against its concentration. It is used to determine the concentration of Iron in the sample by comparing its absorbance to the absorbance of the standard solution. The calibration curve is prepared by measuring the absorbance of standard solutions of Iron with known concentrations.
The Benefits of UV Visible Spectroscopy
UV Visible Spectroscopy is a quick and accurate method for determining the concentration of Iron in vitamin tablets. It is also a non-destructive method, meaning that the sample can be reused for other analyses if required. Furthermore, it is a cost-effective method that requires minimal sample preparation.
Conclusion
Determining the amount of Iron in vitamin tablets is essential to ensure that they are safe for consumption. UV Visible Spectroscopy is a quick and accurate method for determining the concentration of Iron in vitamin tablets. It involves preparing a standard solution of Iron, measuring the absorbance of the sample, and calculating the concentration of Iron in the sample using a calibration curve. UV Visible Spectroscopy is a non-destructive and cost-effective method that requires minimal sample preparation.