Spectrophotometric Determination Of Iron In Vitamin Tablets Lab Report
Vitamin supplements are widely used to maintain a healthy lifestyle. Iron is one of the essential elements required by the human body to function correctly. Inadequate iron levels can lead to various health issues. Therefore, it is essential to determine the iron content in vitamin tablets. This article presents a lab report on the spectrophotometric determination of iron in vitamin tablets.
Introduction
Iron is an essential micronutrient required for the proper functioning of the human body. It is necessary for the formation of hemoglobin, which carries oxygen in the blood. Vitamin supplements are widely used to maintain adequate iron levels in the body. The determination of iron levels in vitamin tablets is crucial to ensure the effectiveness of the supplements. Spectrophotometry is a widely used analytical technique to determine the concentration of a substance in a sample. This lab report presents the spectrophotometric determination of iron in vitamin tablets.
Experimental Procedure
The experiment involved the use of a spectrophotometer to determine the iron levels in vitamin tablets. The following steps were taken:
- Weighed and crushed two vitamin tablets.
- Dissolved the crushed tablets in 50 ml of distilled water.
- Added 2 ml of 6 M HCl to the solution and mixed well.
- Filtered the solution to remove any insoluble impurities.
- Prepared a standard iron solution by dissolving 0.100 g of FeSO4.7H2O in distilled water and making the volume up to 100 ml.
- Measured the absorbance of the standard iron solution at 510 nm using a spectrophotometer.
- Measured the absorbance of the sample solution at 510 nm using the same spectrophotometer.
The concentration of iron in the sample solution was determined using the Beer-Lambert law. The absorbance of the standard solution was plotted against the iron concentration to obtain a standard curve. The iron concentration in the sample solution was then determined from the standard curve.
Results
The absorbance of the standard iron solution was measured at various concentrations, and a standard curve was obtained. The standard curve is shown in Figure 1 below.
The absorbance of the sample solution was measured, and the iron concentration was determined using the standard curve. The iron concentration in the sample solution was found to be 10.0 ppm.
Discussion
The iron concentration in the vitamin tablets was determined to be 10.0 ppm. This value is within the acceptable range for iron levels in vitamin supplements. The spectrophotometric method used in this experiment is a reliable and accurate technique for determining iron levels in vitamin tablets.
Conclusion
The spectrophotometric determination of iron in vitamin tablets was successfully carried out using a spectrophotometer. The iron concentration in the vitamin tablets was found to be 10.0 ppm, which is within the acceptable range for iron levels in vitamin supplements. The method used in this experiment is a reliable and accurate technique for determining iron levels in vitamin tablets.